Why You Should Test Before Taking Hormone Support or NAD+ Supplements

In the world of advanced wellness, supplements like hormone support formulas and NAD+ boosters have become increasingly popular — promising improved energy, better sleep, sharper cognition, muscle growth, and even anti-aging benefits.

But before diving into these powerful interventions, it’s essential to ask one question:
What does your body actually need?

The only way to answer that accurately — and safely — is through comprehensive health testing.

Why Testing Comes First

Your body’s internal chemistry is like a finely tuned orchestra — and hormones and NAD (nicotinamide adenine dinucleotide) are two of the most delicate instruments.

Adding high-impact supplements without knowing your current baseline can throw the system out of balance rather than restore it.

Comprehensive health testing gives you a data-driven foundation, showing:

-How your hormones are performing naturally

-Whether your metabolism and mitochondria are functioning efficiently

-If your nutrient levels (e.g. B3, B12, magnesium, zinc) are optimal for supporting NAD and hormone pathways

-Whether inflammation or stress hormones (like cortisol) are driving symptoms that mimic hormonal imbalance

With that information, you can make targeted, evidence-based choices — instead of taking a “trial and error” approach.

Hormone Support: Not One-Size-Fits-All

Hormones are powerful chemical messengers that influence mood, metabolism, energy, fertility, and more.

But the balance is delicate.

Low energy, brain fog, or weight changes might feel like a hormonal issue — but could actually stem from nutrient deficiencies, inflammation, thyroid imbalance, or blood sugar instability.

Testing first can help identify:

  • Thyroid markers (T3, T4, TSH)

  • Reproductive hormones (oestrogen, progesterone, testosterone, LH, FSH)

  • Cortisol patterns (stress response)

  • Insulin and glucose regulation

  • Inflammatory markers that disrupt hormone signalling

Without testing, using hormone-related supplements could amplify an imbalance rather than correct it.

Science shows: Hormonal changes don’t occur in isolation — they’re connected to inflammation, nutrient status, and metabolic health.

Studies confirm that improving metabolic markers and inflammatory balance can normalise hormone levels naturally.

(Source: National Library of Medicine – Hormone Regulation and Inflammation)

NAD+: The “Cellular Energy” Molecule — Powerful, But Personal

NAD+ is one of the body’s most essential molecules — crucial for cellular energy production, DNA repair, and longevity pathways.


As we age, NAD+ levels decline, leading to fatigue, slower recovery, and cellular aging. NAD+ boosters (like nicotinamide riboside (NR) or nicotinamide mononucleotide (NMN)) are designed to restore that balance.

However, studies show NAD+ metabolism depends on multiple factors, including liver function, oxidative stress, inflammation, and vitamin B status.

If these areas are unbalanced, NAD+ supplementation may not work effectively — or could cause unintended effects like methylation strain or flushing.

(References: Harvard Health – NAD and Aging Research)

By running a comprehensive health test first, you can ensure:

-Your liver and kidney function can handle NAD precursors.
-Your B-vitamin and magnesium levels are optimal for NAD synthesis.
-You’re targeting the right dosage and timing for your biology.

Testing Takes the Guesswork Out

At 100 Checks UK, our Health Profile Test measures 56 biomarkers across:

  • Hormones

  • Glucose and lipids

  • Liver and kidney health

  • Inflammation

  • Nutritional markers

By understanding your full biological picture, you can make intelligent choices about whether hormone support or NAD+ boosters are truly right for you — and how to use them safely and effectively.

Our Full Health Package: Your Personalised Starting Point

For as little as £5 per day, our 6-month Full Health Package includes:

  • Two comprehensive health profile tests (so you can measure improvement)

  • Consultations and personalised reports

  • Tailored guidance on nutrition, lifestyle, and supplementation

  • Six months of foundational supplements, delivered bi-monthly

This gives you a complete roadmap for rebalancing hormones, supporting cellular energy, and truly optimising your health — safely, intelligently, and sustainably.

The Takeaway

Before reaching for powerful supplements like NAD+ boosters or hormone support, take a step back and test.

Your data holds the answers — and once you know your unique biology, you can work with it, not against it.

Health testing is not just prevention — it’s precision.
And when it comes to your hormones, longevity, and vitality, precision is power.

Back to blog